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Copyright @ Pol J Cosmetol
Pol J Cosmetol 2009, 12(4): 267-274pl,en  Application of oil-soluble antioxidants during UV stressLidia Kryczkowska 1/, Oksana Strus 1/, Anna Bielińska 1/, Wiktor Preżdo 2/ 1/ Department of Biotechnology, Kharkiv Polytechnic Institute, 21 Frunze Street, 61002 Kharkiv, Ukraine 2/ Department of Environmental Protection, Stanisław Staszic University, Poland Summary Introduction. Antioxidants inactivate and neutralize active forms of oxygen and free radicals which form in the skin exposed to the UV radiation. Therefore antioxidants can be used in therapy and prevention. The most known antioxidant is α-tocopherol which protects lipid cell membranes. Aim. To prepare a new cosmetic remedy with photo-protective and antioxidative properties on the basis of microbiological carotene. Material and methods. The study object was microbiological carotene extracted from biomass of the Blakeslea trispora micro-fungus, fatty cream base and carotene-containing cream. The photo-protective properties of fatty-based cream and antioxidative property of carotene was studied. Results. The study was an attempt to compare the influence of both preparation samples on the index of free radical oxidation and antiradical protection of some body tissues under stress influence (UV radiation). The introduction of α-tocopherol 30 min. before the UV stress leads to a decrease in cholesterol content and an increase in phospholipids. The introduction of carotene with biologically active substances similarly decreases antioxidative activity of the lipid extracts, but more than the introduction of α-tocopherol alone. Conclusions. The mechanisms of antioxidative influence of carotene in interaction with fat-soluble vitamins are complex and should be thoroughly investigated. The effect of natural oxidants in natural oils on the base of which the carotene samples were prepared should be researched. A great amount of α-tocoferol and phospholipids, which play an important role in the defense of lipids against oxidation and its products, can be observed in sunflower and amarant oils. The carotene antioxidative activity showing high biological accessibility can predetermine its application in antistress therapy if a decrease in free-radical activity processes is necessary. Key words: antioxidants, UV radiation, beta-carotene, alpha-tocopherol, free radicals |