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Copyright @ Pol J Cosmetol
Pol J Cosmetol 2017, 20(1): 63-66pl  Spectroscopic comparative analysis of the antioxidative properties of extracts from herb Lycopodium (Lycopodium clavatum L.)Małgorzata Stec, Paweł Ramos, Barbara Pilawa Katedra i Zakład Biofizyki, Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej w Sosnowcu, Śląski Uniwersytet Medyczny w Katowicach Summary Introduction. Herb of Lycopodium clavatum L. in medicine and cosmetology can be used. Antioxidative properties of different water extracts from Lycopodium clavatum were compared in the aim to check their eventual applications in cosmetology. Interactions of infusion and decoction from Lycopodium clavatum with DPPH free radicals were examined. The value and the kinetics of these interactions were determined. Aim. The aim of this study was to determine whether the aqueous extracts of the herb Lycopodium clavatum L. have antioxidant properties. Material and methods. The infusion of the Lycopodium clavatum herb after 15 minutes treatment with boiling water was obtained. The decoction after boiling the herb for 15 minutes and leaving covered for a further 15 minutes was obtained. The experimental method was electron paramagnetic resonance spectroscopy with the microwave frequency of 9.3 GHz. EPR spectra of DPPH free radicals were measured by the use of spectrometer of Radiopan Firm (Poznań) and the numerical system of data acquisition of Jagmar Firm (Kraków). Results. Amplitdes of EPR lines of DPPH free radicals decreased as the result of interactions with infusion and decoction of Lycopodium clavatum, what confirmed their antioxidative character. Conclusion. Kinetics of interations of these infusions and decoctions of the herb Lycopodium clavatum with DPPH free radicals was similar. Decoction of Lycopodium clavatum L. was the strongest antioxidant relative to the infusion. Key words: Lycopodium clavatum, extract, free radicals, antioxidants, EPR spectroscopy |