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Copyright @ Pol J Cosmetol
Pol J Cosmetol 2011, 14(1): 62-68pl  Challenge tests of cosmetic products in a production plant laboratory - a comparison of internal standards and ISO/CD 11930Małgorzata Gniewosz, Agata Stobnicka, Tomasz Stankiewicz Katedra Biotechnologii, Mikrobiologii i Oceny Żywności, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie Summary Introduction. Health safety of cosmetic products depends on them microbiological quality. Therefore, cosmetics, leaving the production plant must not only meet the appropriate standards for their microbiological status, but also must be properly preserved, in order to overcome secondary infections associated with their use. To verify the effectiveness of the applied preservative used in cosmetics, the production plant laboratory should carry out challenge tests, which provide information about the efficacy of the preservatives. Aim. The aim of this study was to compare the methods of internal production plant challenge tests with the method contained in the draft of the new standard for the cosmetics industry ISO/CD 11930, for the same tests. Challenge tests were carried out in selected cosmetic products and compared the two methodologies results. Materials and methods. The study used samples of finished cosmetic products, which introduced a suspension of microorganisms isolated from surfaces in production plant (according to internal standards) and from ATCC collection (according to ISO/CD 11930). Results. It was found that the impact on the results of challenge tests was the number of cells in the inoculum, which was introduced to the samples of cosmetics, and the applied strain of Burkholderia cepacia (strain ATCC 25416 or isolated in production plant), while the number of bacteria in the inoculum was 1×108 jtk/cm3. Conclusions. In the cases studied, the proceedings in accordance with the draft standard ISO/CD 11930 resulted in a greater number of samples that could be marketed. Key words: challenge tests, ISO/CD 11930, preservatives |