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Copyright @ Pol J Cosmetol
Pol J Cosmetol 2024, 27(2): 97-105pl  Micellar solubilization of cholesterol by aqueous solutions of ginsenosides contained in the extract of Panax ginsengMarian M. Zgoda 1/, Sławomira Nowak 2/, Zbigniew Marczyński 3/, Łukasz Kuźma 4/, Magdalena Markowicz-Piasecka 3/, Beata Skibska 3/ 1/ Zakład Technologii Postaci Leku, Katedra Farmacji Stosowanej Uniwersytetu Medycznego w Łodzi (prof. senior) 2/ Katedra i Zakład Farmakognozji Uniwersytetu Medycznego w Łodzi 3/ Zakład Farmacji Aptecznej, Katedra Farmacji Stosowanej Uniwersytetu Medycznego w Łodzi 4/ Zakład Biologii i Botaniki Farmaceutycznej, Katedra Farmakognozji Uniwersytetu Medycznego w Łodzi Summary Introduction. The structure of the ginsenoside molecule of the Panax ginseng extract components, based on the lipophilic structure of protopanaxadiol and protopanaxatriol and glycosidic bonds to sugar alcohols, determines their original phytotherapeutic properties, especially in complex preparations that support the growth of the body immunity and its regeneration. The contemporary profile of preformulation studies and available literature were the inspiration to calculate the thermodynamic parameters of ginsenosides and, above all, to determine their surface activity and cholesterol solubilization capacity. Aim. The aim of the studies was to estimate the thermodynamic parameters of ginsenosides in water and ethanol and above all, to investigate the surface activity of extracts in model acceptor fluids and their solubilizing abilities in relation to heterogeneous morphological forms of cholesterol. Material and methods. The subject of the study was a dried extract from the roots of Panax ginseng (Panax ginseng extr. siccum) containing ginsenosides, surface-active compounds in pharmacopoeial acceptor fluids. The physicochemical and solubilization properties of the extract solutions were determined using pharmacopoeial methods. Results. Thermodynamic parameters were calculated for ginsenoside structures, which allow for the estimation of the solubility parameter and the solubility level HLBRequ. Ideal solubility was calculated for lipophilic structures of protopanaxadiol and -triol. The surface activity of the extract in model acceptor fluids was examined and, above all, its solubilizing capacity for heterogeneous morphological forms of cholesterol was determined. The thermodynamic stability of the adduct was calculated after micellar solubilization of cholesterol. Conclusions. The compatible structure of protopanaxadiol and -triol in the ginsenoside molecule with respect to cholesterol makes them not only effective solubilizers of heterogeneous morphological forms of its structure and surprisingly, the cholesterol micellar adduct is characterized by greater thermodynamic stability than the ginsenoside micelle. Key words: Panax ginseng, ginsenosides, protopanaxadiol, protopanaxatriol, solubility, solubilization, cholesterol |